Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II

Serial crystallography (SX) enables the determination of room-temperature structures with minimal radiation damage. The photon flux pink beam 1.2% bandwidth (BW) is one order higher than that monochromatic from a silicon crystal monochromator, and energy resolution BW enough to solve structure; therefore, it useful use for time-resolved serial synchrotron (SSX). Here, we demonstrate pink-beam crystallographic study at 1C beamline Pohang Light Source II. Lysozyme crystals embedded in beef tallow injection matrix were delivered through syringe into X-ray interaction point. Pink-beam SSX was performed different exposure positions stream (center edge) times (50 100 ms). All lysozyme successfully determined high 1.7 Å. Background analysis showed diffraction data exposed edge could improve signal-to-noise ratio. comprehensively compared. collection strategy will be helpful further experiments their applications.